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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/02/2021 |
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Actualizado : |
27/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IRIARTE, W.; MURCHIO, S.; RODRIGUEZ, P.; CABRERA, D.; SORIA, J.; PISANO, J.; ZOPPOLO, R.; DALLA RIZZA, M. |
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Afiliación : |
WANDA IRIARTE GRECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ANDRES RODRIGUEZ BRUNO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE RAUL SORIA BARAIBAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO CESAR PISANO CARBAJAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Pear accession fingerprinting through microsatellite markers in Uruguay. [Conference paper] |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Acta Horticulturae, February 2021, N°1303, p. 91-100. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.14 |
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ISBN : |
978-94-62613-01-0 |
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ISSN : |
0567-7572 (print); 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2021.1303.14 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. |
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Contenido : |
Abstract.
A selection of 11 microsatellite markers (SSRs) reported for fingerprinting germplasm collections of Pyrus spp. was used to genotype a collection of cultivars and rootstocks. This set of markers included the "minimum core" established by Evans et al. (2009). Cultivars with known origin ('Williams', 'Abbé Fétel' and 'Doyenné du Comice') were included as reference accessions. The comparison was performed among 44 accessions collected from old pear plantations with different commercial origins and date of introduction to the country. The aim of this study was to genotype pear accessions that were introduced in Uruguay to evaluate the genetic variability among clones and pear rootstocks. The selected markers proved to be effective for variability discrimination in all the accessions having 6 to 12 alleles per locus. The most informative markers were CH01d09, CH02b10 and GD96. Within cultivars, the observed variability among the 13 'Williams' clones could respond to the numerous introductions from different origins that were performed throughout the years of pear production in the country. Although the accessions were grouped in clearly defined clusters as expected before the analysis, they showed variability within cultivars. The accession 00LBPrSJ is a rootstock collected from the locality of San José, selected because of its medium to low vigor that leads to medium-sized fruit trees. This accession showed a particular molecular pattern profile characterized by unique alleles that make it genetically distant from other accessions. The above mentioned phenotypic feature represents a very appealing condition, that is suitable for the current cultivation practices overcoming incompatibility problems.
@ International Society for Horticultural Science. MenosAbstract.
A selection of 11 microsatellite markers (SSRs) reported for fingerprinting germplasm collections of Pyrus spp. was used to genotype a collection of cultivars and rootstocks. This set of markers included the "minimum core" established by Evans et al. (2009). Cultivars with known origin ('Williams', 'Abbé Fétel' and 'Doyenné du Comice') were included as reference accessions. The comparison was performed among 44 accessions collected from old pear plantations with different commercial origins and date of introduction to the country. The aim of this study was to genotype pear accessions that were introduced in Uruguay to evaluate the genetic variability among clones and pear rootstocks. The selected markers proved to be effective for variability discrimination in all the accessions having 6 to 12 alleles per locus. The most informative markers were CH01d09, CH02b10 and GD96. Within cultivars, the observed variability among the 13 'Williams' clones could respond to the numerous introductions from different origins that were performed throughout the years of pear production in the country. Although the accessions were grouped in clearly defined clusters as expected before the analysis, they showed variability within cultivars. The accession 00LBPrSJ is a rootstock collected from the locality of San José, selected because of its medium to low vigor that leads to medium-sized fruit trees. This accession showed a particular molecular pattern profile characterized by unique... Presentar Todo |
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Palabras claves : |
Average linkage; Genotyping; SSR. |
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Thesagro : |
PYRUS SPP. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02937naa a2200301 a 4500
001 1061738
005 2021-04-27
008 2021 bl uuuu u00u1 u #d
020 $a978-94-62613-01-0
022 $a0567-7572 (print); 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2021.1303.14$2DOI
100 1 $aIRIARTE, W.
245 $aPear accession fingerprinting through microsatellite markers in Uruguay. [Conference paper]$h[electronic resource]
260 $c2021
500 $aArticle history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein.
520 $aAbstract. A selection of 11 microsatellite markers (SSRs) reported for fingerprinting germplasm collections of Pyrus spp. was used to genotype a collection of cultivars and rootstocks. This set of markers included the "minimum core" established by Evans et al. (2009). Cultivars with known origin ('Williams', 'Abbé Fétel' and 'Doyenné du Comice') were included as reference accessions. The comparison was performed among 44 accessions collected from old pear plantations with different commercial origins and date of introduction to the country. The aim of this study was to genotype pear accessions that were introduced in Uruguay to evaluate the genetic variability among clones and pear rootstocks. The selected markers proved to be effective for variability discrimination in all the accessions having 6 to 12 alleles per locus. The most informative markers were CH01d09, CH02b10 and GD96. Within cultivars, the observed variability among the 13 'Williams' clones could respond to the numerous introductions from different origins that were performed throughout the years of pear production in the country. Although the accessions were grouped in clearly defined clusters as expected before the analysis, they showed variability within cultivars. The accession 00LBPrSJ is a rootstock collected from the locality of San José, selected because of its medium to low vigor that leads to medium-sized fruit trees. This accession showed a particular molecular pattern profile characterized by unique alleles that make it genetically distant from other accessions. The above mentioned phenotypic feature represents a very appealing condition, that is suitable for the current cultivation practices overcoming incompatibility problems. @ International Society for Horticultural Science.
650 $aPYRUS SPP
653 $aAverage linkage
653 $aGenotyping
653 $aSSR
700 1 $aMURCHIO, S.
700 1 $aRODRIGUEZ, P.
700 1 $aCABRERA, D.
700 1 $aSORIA, J.
700 1 $aPISANO, J.
700 1 $aZOPPOLO, R.
700 1 $aDALLA RIZZA, M.
773 $tActa Horticulturae, February 2021, N°1303, p. 91-100. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.14
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Registro original : |
INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/01/2020 |
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Actualizado : |
21/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASAUX, M.L.; CAFFARENA, D.; SCHILD, C.; GIANNITTI, F.; RIET-CORREA, F.; FRAGA, M. |
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Afiliación : |
MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Antibiotic resistance in Salmonella enterica isolated from dairy calves in Uruguay. (Veterinary Microbiology - Short Communication) |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Brazilian Journal of Microbiology, 1 October 2019, Volume 50, Issue 4, Pages 1139-1144. Doi: 10.1007/s42770-019-00151-w |
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ISSN : |
1517-8382 |
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DOI : |
10.1007/s42770-019-00151-w |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 22 January 2019 / Accepted: 4 September 2019.
The online version of this article contains supplementary material. |
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Contenido : |
ABSTRACT.
Salmonella enterica is an important animal and human pathogen that can cause enteritis and septicaemia in calves. Generally, antibiotics are prescribed for the treatment of salmonellosis in dairy calves. Here, we report the isolation of antibiotic resistant S. enterica serotypes from calves, including multidrug-resistant isolates. A total of 544 faecal samples from live healthy and diarrheic dairy calves from 29 commercial dairy farms and organ samples from 19 deceased calves that succumbed to salmonellosis in 12 commercial dairy farms in Uruguay were processed for selective S. enterica culture. In total, 41 isolates were serotyped, and susceptibility to 14 antibiotics, from 9 classes of compounds, was evaluated by disk-diffusion test. The minimum inhibitory concentration (MIC) was determined by microdilution. Salmonella Typhimurium was the most frequent serotype, followed by S. Dublin and S. Anatum. Whether determined by diffusion assay or microdilution, resistance to tetracycline, streptomycin and ampicillin were the most frequently pattern found. Based on MIC, 5 isolates were resistant to at least one antibiotic, 21 were resistant to 2 antibiotics, and 14 were multidrug-resistant (resistant to at least one antibiotic in 3 different categories of antibiotics). Eleven different resistance patterns were found. Multidrug resistance in S. enterica is a concern for animal and public health not only because of its zoonotic potential but also due to the possibility of transfer resistance determinants to other bacterial genera. This represents the first report of the antibiotic resistance in S. enterica in dairy farms in Uruguay.
© 2019, Sociedade Brasileira de Microbiologia. MenosABSTRACT.
Salmonella enterica is an important animal and human pathogen that can cause enteritis and septicaemia in calves. Generally, antibiotics are prescribed for the treatment of salmonellosis in dairy calves. Here, we report the isolation of antibiotic resistant S. enterica serotypes from calves, including multidrug-resistant isolates. A total of 544 faecal samples from live healthy and diarrheic dairy calves from 29 commercial dairy farms and organ samples from 19 deceased calves that succumbed to salmonellosis in 12 commercial dairy farms in Uruguay were processed for selective S. enterica culture. In total, 41 isolates were serotyped, and susceptibility to 14 antibiotics, from 9 classes of compounds, was evaluated by disk-diffusion test. The minimum inhibitory concentration (MIC) was determined by microdilution. Salmonella Typhimurium was the most frequent serotype, followed by S. Dublin and S. Anatum. Whether determined by diffusion assay or microdilution, resistance to tetracycline, streptomycin and ampicillin were the most frequently pattern found. Based on MIC, 5 isolates were resistant to at least one antibiotic, 21 were resistant to 2 antibiotics, and 14 were multidrug-resistant (resistant to at least one antibiotic in 3 different categories of antibiotics). Eleven different resistance patterns were found. Multidrug resistance in S. enterica is a concern for animal and public health not only because of its zoonotic potential but also due to the possibility of t... Presentar Todo |
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Palabras claves : |
Antibiotic resistance; Dairy calves; PLATAFORMA SALUD ANIMAL; Salmonella Anatum; Salmonella Dublin; Salmonella Typhimurium. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02796naa a2200289 a 4500
001 1060657
005 2020-01-21
008 2019 bl uuuu u00u1 u #d
022 $a1517-8382
024 7 $a10.1007/s42770-019-00151-w$2DOI
100 1 $aCASAUX, M.L.
245 $aAntibiotic resistance in Salmonella enterica isolated from dairy calves in Uruguay. (Veterinary Microbiology - Short Communication)$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 22 January 2019 / Accepted: 4 September 2019. The online version of this article contains supplementary material.
520 $aABSTRACT. Salmonella enterica is an important animal and human pathogen that can cause enteritis and septicaemia in calves. Generally, antibiotics are prescribed for the treatment of salmonellosis in dairy calves. Here, we report the isolation of antibiotic resistant S. enterica serotypes from calves, including multidrug-resistant isolates. A total of 544 faecal samples from live healthy and diarrheic dairy calves from 29 commercial dairy farms and organ samples from 19 deceased calves that succumbed to salmonellosis in 12 commercial dairy farms in Uruguay were processed for selective S. enterica culture. In total, 41 isolates were serotyped, and susceptibility to 14 antibiotics, from 9 classes of compounds, was evaluated by disk-diffusion test. The minimum inhibitory concentration (MIC) was determined by microdilution. Salmonella Typhimurium was the most frequent serotype, followed by S. Dublin and S. Anatum. Whether determined by diffusion assay or microdilution, resistance to tetracycline, streptomycin and ampicillin were the most frequently pattern found. Based on MIC, 5 isolates were resistant to at least one antibiotic, 21 were resistant to 2 antibiotics, and 14 were multidrug-resistant (resistant to at least one antibiotic in 3 different categories of antibiotics). Eleven different resistance patterns were found. Multidrug resistance in S. enterica is a concern for animal and public health not only because of its zoonotic potential but also due to the possibility of transfer resistance determinants to other bacterial genera. This represents the first report of the antibiotic resistance in S. enterica in dairy farms in Uruguay. © 2019, Sociedade Brasileira de Microbiologia.
653 $aAntibiotic resistance
653 $aDairy calves
653 $aPLATAFORMA SALUD ANIMAL
653 $aSalmonella Anatum
653 $aSalmonella Dublin
653 $aSalmonella Typhimurium
700 1 $aCAFFARENA, D.
700 1 $aSCHILD, C.
700 1 $aGIANNITTI, F.
700 1 $aRIET-CORREA, F.
700 1 $aFRAGA, M.
773 $tBrazilian Journal of Microbiology, 1 October 2019, Volume 50, Issue 4, Pages 1139-1144. Doi: 10.1007/s42770-019-00151-w
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